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大鼠肝微粒体组分中肌苷-5'-二磷酸酶的生化与细胞化学定位

Biochemical and cytochemical localization of inosine-5'-diphosphatase in rat liver microsomal fractions.

作者信息

Little J S

出版信息

Biochem J. 1984 Mar 1;218(2):501-10. doi: 10.1042/bj2180501.

DOI:10.1042/bj2180501
PMID:6143555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1153366/
Abstract

A procedure has been developed for the biochemical and cytochemical localization of inosine-5'-diphosphatase (IDPase) in rat liver microsomal fractions. The concentration of Pb2+ ions used in the cytochemical incubation medium inhibited IDPase by 32%. IDP formed during the incubation was not hydrolysed further to inosine, and no significant non-enzymic hydrolysis of substrate occurred. When microsomal fractions were incubated cytochemically for IDPase and separated from unchanged substrate under conditions such that the microsomal membrane was not permeable to EDTA, approx. 80% of the reaction product was solubilized by EDTA. In the electron microscope, lead precipitate was observed on the cytoplasmic side (outside) of a major fraction of the vesicles and on the cisternal side (inside) of others. After extraction with EDTA, the lead precipitate was observed only on the cisternal side. Since it was shown that phosphate is trapped on the same side of the membrane as it is released, it is concluded that IDPase can release phosphate on either the cisternal or the cytoplasmic side of the microsomal membrane.

摘要

已开发出一种用于在大鼠肝微粒体组分中对肌苷-5'-二磷酸酶（IDPase）进行生化和细胞化学定位的方法。细胞化学孵育培养基中使用的Pb2+离子浓度使IDPase活性抑制了32%。孵育过程中形成的IDP不会进一步水解为肌苷，且底物未发生明显的非酶促水解。当微粒体组分在细胞化学条件下孵育IDPase并在微粒体膜对EDTA不可渗透的条件下与未反应的底物分离时，约80%的反应产物可被EDTA溶解。在电子显微镜下，在大部分囊泡的细胞质侧（外侧）和其他囊泡的潴泡侧（内侧）观察到铅沉淀。用EDTA提取后，仅在潴泡侧观察到铅沉淀。由于已表明磷酸盐在其释放的膜的同一侧被截留，因此得出结论，IDPase可在微粒体膜的潴泡侧或细胞质侧释放磷酸盐。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/7812fb0702d5/biochemj00332-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/5bf9f0fb45c0/biochemj00332-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/5f3c8ef48dca/biochemj00332-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/2529a108e3a0/biochemj00332-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/23e0fe2a3b76/biochemj00332-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/7812fb0702d5/biochemj00332-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/5bf9f0fb45c0/biochemj00332-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/5f3c8ef48dca/biochemj00332-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/2529a108e3a0/biochemj00332-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/23e0fe2a3b76/biochemj00332-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d000/1153366/7812fb0702d5/biochemj00332-0226-a.jpg

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本文引用的文献

1
Phosphatases of liver. I. Glucose-6-phosphatase.肝脏磷酸酶。I. 葡萄糖-6-磷酸酶。
J Biol Chem. 1950 Jun;184(2):647-59.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE LOCALIZATION OF PHOSPHATASE ACTIVITIES AT THE LEVEL OF ULTRASTRUCTURE.超微结构水平上磷酸酶活性的定位
J Histochem Cytochem. 1964 Feb;12:72-95. doi: 10.1177/12.2.72.
4
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
5
A microsomal nucleoside diphosphatase.一种微粒体核苷二磷酸酶。
J Biol Chem. 1963 Feb;238:710-6.
6
The localization of adenosine triphosphatase in liver: in situ staining and cell fractionation studies.肝脏中三磷酸腺苷酶的定位：原位染色及细胞分级分离研究
J Histochem Cytochem. 1958 Jan;6(1):61-71. doi: 10.1177/6.1.61.
7
Ultraviolet absorption spectra of adenosine-5'-triphosphate and related 5'-ribonucleotides.腺苷 - 5'-三磷酸及相关5'-核糖核苷酸的紫外吸收光谱。
Arch Biochem Biophys. 1956 Jun;62(2):253-64. doi: 10.1016/0003-9861(56)90123-0.
8
A major protein which spans the human erythrocyte membrane.一种贯穿人类红细胞膜的主要蛋白质。
J Mol Biol. 1971 Jul 28;59(2):351-7. doi: 10.1016/0022-2836(71)90055-6.
9
Cytochemical localization of 5'-nucleotidase in subcellular fractions isolated from rat liver. I. The origin of 5'-nucleotidase activity in microsomes.大鼠肝脏分离的亚细胞组分中5'-核苷酸酶的细胞化学定位。I. 微粒体中5'-核苷酸酶活性的来源。
J Cell Biol. 1972 Mar;52(3):542-58. doi: 10.1083/jcb.52.3.542.
10
The localization of spectrin on the inner surface of human red blood cell membranes by ferritin-conjugated antibodies.通过铁蛋白偶联抗体将血影蛋白定位在人红细胞膜的内表面。
J Cell Biol. 1971 Oct;51(1):265-72. doi: 10.1083/jcb.51.1.265.