Granata A R, Sved A F, Reis D J
Brain Res Bull. 1984 Jan;12(1):5-9. doi: 10.1016/0361-9230(84)90207-7.
In anesthetized and paralyzed rats, using a push-pull perfusion technique, we examined the effect of bilateral vagal stimulation on the release of L-[3H] glutamic acid (L- [3H] Glu) from the nucleus tractus solitarius (NTS), after preloading the tissue either with L-[3H] Glu or L-[3H] glutamine (L- [3H] Gln). Vagal stimulation sufficient to produce a maximum fall of arterial pressure (AP) evoked release of L- [3H] Glu from the NTS when the tissue was preloaded with either 3H-Glu or 3H-Gln, and of D-[3H] aspartic acid (D-[3H] Asp) when this stable Glu analogue was used to preloaded with either 3H-Glu or 3H-Gln, and of D-[3H] precursor L-Gln is a good marker of the releasable pool of L-Glu in vivo and are consistent with the hypothesis that L-[3H] Glu is a neurotransmitter in the NTS, mediating the vasodepressor response from cardiopulmonary mechanoreceptors.
在麻醉和麻痹的大鼠中，我们采用推挽灌注技术，在预先用L-[3H]谷氨酸（L-[3H]Glu）或L-[3H]谷氨酰胺（L-[3H]Gln）对组织进行预加载后，研究了双侧迷走神经刺激对孤束核（NTS）释放L-[3H]谷氨酸的影响。当组织用3H-Glu或3H-Gln预加载时，足以使动脉血压（AP）最大下降的迷走神经刺激会诱发NTS释放L-[3H]Glu；当使用这种稳定的Glu类似物D-[3H]天冬氨酸（D-[3H]Asp）预加载3H-Glu或3H-Gln时，会诱发其释放；而当使用D-[3H]前体L-Gln预加载时，也会诱发其释放。L-Gln是体内可释放的L-Glu池的良好标志物，这与L-[3H]Glu是NTS中的神经递质、介导心肺机械感受器的血管减压反应这一假设相一致。
