Skinner M, Marbrook J
Cell Immunol. 1984 May;85(2):519-30. doi: 10.1016/0008-8749(84)90264-8.
The induction of cytotoxic T lymphocytes by concanavalin A has been analyzed under conditions of limit dilution. The dose-response curves deviate from linearity in a way that has been interpreted as revealing successive zones of suppression as the cell concentration was increased. The magnitude of suppression was influenced by both the concentration of concanavalin A and the amount of T cell growth factors added to the culture. These regulatory events involve the cytotoxic T cell clones produced by (CBA X DBA)F1 spleen cells which are detected by DBA mastocytoma (P815) targets at a maximum detectable frequency of 1 in 2000 cells. Similar multiphase dose-response data were also obtained with syngeneic and allogeneic combinations with the same target cell. It is suggested that the successive zones of suppression and activation are a consequence of the relative frequencies of CTL-P, suppressive and helper cells, and the ease with which the cells are activated in limit dilution cultures. The experimental approach illustrates how CTL production can be manipulated to study the balance of signals required to control effector cell production.
在极限稀释条件下分析了伴刀豆球蛋白A对细胞毒性T淋巴细胞的诱导作用。剂量反应曲线偏离线性，这种方式被解释为随着细胞浓度增加揭示出连续的抑制区域。抑制程度受伴刀豆球蛋白A浓度和添加到培养物中的T细胞生长因子量的影响。这些调节事件涉及由（CBA×DBA）F1脾细胞产生的细胞毒性T细胞克隆，这些克隆由DBA肥大细胞瘤（P815）靶细胞检测，最大可检测频率为每2000个细胞中有1个。在与相同靶细胞的同基因和异基因组合中也获得了类似的多相剂量反应数据。有人提出，连续的抑制和激活区域是细胞毒性T淋巴细胞前体（CTL-P）、抑制细胞和辅助细胞相对频率以及细胞在极限稀释培养中被激活的难易程度的结果。该实验方法说明了如何操纵CTL的产生来研究控制效应细胞产生所需信号的平衡。
