Knoop F C, Thomas D D
Infect Immun. 1984 May;44(2):406-8. doi: 10.1128/iai.44.2.406-408.1984.
The effect of lodoxamide tromethamine, a calcium antagonist, on intestinal fluid accumulation induced by Escherichia coli heat-stable (ST) and Vibrio cholerae (CT) enterotoxins in infant mice was investigated. The simultaneous administration of lodoxamide with ST or CT enterotoxin resulted in a significant (P less than 0.01) inhibition of the intestinal fluid response. A minimum concentration of 10(-7) or 10(-8)M lodoxamide caused an inhibition (P less than 0.01) of the ST- or CT-mediated fluid response, respectively. Treatment of infant mice with buffer or drug alone did not result in fluid accumulation. A significant inhibition of ST and CT enterotoxic activities was also observed when lodoxamide was administered 30 min before (P less than 0.02) or 30 min after (P less than 0.01) toxin challenge. These data suggest that calcium may be important in the ST- or CT-mediated induction of fluid accumulation. Further studies on the potential use of lodoxamide tromethamine in both the prophylaxis and treatment of diarrheal disease appear warranted.
研究了钙拮抗剂三甲曲沙洛度胺对婴儿小鼠中由大肠杆菌热稳定（ST）毒素和霍乱弧菌（CT）肠毒素诱导的肠液积聚的影响。将洛度胺与ST或CT肠毒素同时给药可导致对肠液反应的显著（P小于0.01）抑制。最低浓度为10^(-7)或10^(-8)M的洛度胺分别导致对ST或CT介导的液体反应的抑制（P小于0.01）。仅用缓冲液或药物处理婴儿小鼠不会导致液体积聚。当在毒素攻击前30分钟（P小于0.02）或后30分钟（P小于0.01）给予洛度胺时，也观察到对ST和CT肠毒素活性的显著抑制。这些数据表明钙在ST或CT介导的液体积聚诱导中可能很重要。进一步研究三甲曲沙洛度胺在腹泻病预防和治疗中的潜在用途似乎是有必要的。
