Igarashi T, Satoh T, Ueno K, Kitagawa H
J Pharmacobiodyn. 1983 Dec;6(12):941-9. doi: 10.1248/bpb1978.6.941.
Total glutathione (GSH and GSSG) level, and the activities of gamma-glutamylcysteine synthetase, gamma-glutamyltranspeptidase (gamma-GTP), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) in the liver were investigated in rats, mice, guinea pigs and hamsters. Hepatic GSH level in rats, mice, guinea pigs and hamsters were 7.1, 7.8, 3.5 and 5.4 mM, respectively. The lower level of GSH in guinea pigs seems to be in part attributed to the higher activity of hepatic gamma-GTP, an enzyme which catalyzes GSH breakdown. Moreover, a marked species difference in the activities of GST, GSH-Px and GR was also observed. A 48 h-fasting resulted in a decrease of GSH and GSSG levels in rats, mice and guinea pigs, but not in hamsters. In addition, both nicotineamide adenine dinucleotide phosphate- and ascorbate-dependent lipid peroxidation produced by 9000 X g supernatant fraction in fasted animal species occurred most highly in the rat followed by hamster and guinea pig, and almost undetectable in mice. Thus, it suggests that the occurrence of lipid peroxidation in fasted animals may not be related to the hepatic GSH level, and rather, a lack of occurrence of lipid peroxidation in fasted mice may be due to the increased activity of GSH-Px activity.
在大鼠、小鼠、豚鼠和仓鼠中研究了肝脏中总谷胱甘肽（GSH和GSSG）水平，以及γ-谷氨酰半胱氨酸合成酶、γ-谷氨酰转肽酶（γ-GTP）、谷胱甘肽S-转移酶（GST）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽还原酶（GR）的活性。大鼠、小鼠、豚鼠和仓鼠肝脏中的GSH水平分别为7.1、7.8、3.5和5.4 mM。豚鼠中较低的GSH水平似乎部分归因于肝脏γ-GTP活性较高，γ-GTP是一种催化GSH分解的酶。此外，还观察到GST、GSH-Px和GR活性存在明显的物种差异。禁食48小时导致大鼠、小鼠和豚鼠的GSH和GSSG水平下降，但仓鼠没有。此外，禁食动物的9000×g上清液部分产生的烟酰胺腺嘌呤二核苷酸磷酸和抗坏血酸依赖性脂质过氧化在大鼠中最高，其次是仓鼠和豚鼠，在小鼠中几乎检测不到。因此，这表明禁食动物中脂质过氧化的发生可能与肝脏GSH水平无关，相反，禁食小鼠中脂质过氧化未发生可能是由于GSH-Px活性增加。
