Verdonck L F, de Gast G C
Lancet. 1984 Apr 28;1(8383):932-5. doi: 10.1016/s0140-6736(84)92391-2.
Peripheral-blood T cell subsets and functions were studied in 14 patients with malignancies treated with high-dose chemotherapy and radiotherapy followed by autologous bone-marrow transplantation (BMT). 8 CMV-positive patients (6 with latent infections and 2 with a primary infection) showed an inversion of the OKT4/OKT8 ratio caused by an increase in OKT8+ T cells and a decrease in OKT4+ T cells. This was accompanied by a pronounced increase in the percentage of HLA-DR + T cells, and functionally by increased suppressor T cell activity and decreased helper T cell activity and T cell proliferation. These alterations in T cell subsets and functions were not observed in 6 patients who were kept CMV-negative by a deliberate transfusion policy. In the 6 patients helper T cell activity and T cell proliferation capacity recovered well after day 30. Differences between the two groups were most striking 60 days after BMT. This study suggests that CMV infection, whether primary or secondary, is a major cause of T cell alterations after (autologous) BMT.
对14例接受大剂量化疗和放疗后进行自体骨髓移植（BMT）的恶性肿瘤患者的外周血T细胞亚群及功能进行了研究。8例巨细胞病毒（CMV）阳性患者（6例为潜伏感染，2例为原发感染）表现为OKT4/OKT8比值倒置，原因是OKT8 + T细胞增加而OKT4 + T细胞减少。这伴随着HLA - DR + T细胞百分比的显著增加，在功能上表现为抑制性T细胞活性增加、辅助性T细胞活性降低以及T细胞增殖减少。通过精心的输血策略保持CMV阴性的6例患者未观察到T细胞亚群及功能的这些改变。在6例患者中，辅助性T细胞活性和T细胞增殖能力在第30天后恢复良好。两组之间的差异在BMT后60天最为显著。本研究表明，CMV感染，无论是原发还是继发，都是（自体）BMT后T细胞改变的主要原因。
