用于生产苏云金芽孢杆菌以色列亚种原始粉末的培养基。
Medium for the production of primary powder of Bacillus thuringiensis subsp. israelensis.
作者信息
Obeta J A, Okafor N
出版信息
Appl Environ Microbiol. 1984 Apr;47(4):863-7. doi: 10.1128/aem.47.4.863-867.1984.
Five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of Bacillus thuringiensis subsp. israelensis. Bacterial powders prepared from the broth cultures were assayed against the larvae of Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae. A standard primary powder of B. thuringiensis subsp. israelensis (IPS78) was included in the assay for comparison. Good growth was obtained in all the media, and all powders were effective against the three types of mosquito larvae. The powder containing ground seeds of Voandzeia subterranean was the most effective and compared favorably with the standard (IPS78). The concentrations required to kill 50% of the larvae of Aedes aegypti, C. quinquefasciatus, and Anopheles gambiae were 1.13 X 10(-2) +/- 1.79 X 10(-3), 1.83 X 10(-2) +/- 2.55 X 10(-3), and 2.25 X 10(-2) +/- 1.88 X 10(-3) micrograms/ml, respectively. This investigation shows that the medium containing V. subterranean can be used for the production of B. thuringiensis subsp. israelensis primary powder.
对由五种豆科植物种子、干牛血和矿物盐配制而成的五种培养基，进行了苏云金芽孢杆菌以色列亚种杀虫特性生长及产生情况的评估。用肉汤培养物制备的菌粉针对埃及伊蚊、致倦库蚊和冈比亚按蚊的幼虫进行了测定。测定中纳入了苏云金芽孢杆菌以色列亚种（IPS78）的标准原粉用于比较。在所有培养基中菌生长良好，所有菌粉对三种类型的蚊虫幼虫均有效。含非洲豇豆磨碎种子的菌粉最有效，与标准品（IPS78）相比效果良好。杀死50%埃及伊蚊、致倦库蚊和冈比亚按蚊幼虫所需的浓度分别为1.13×10⁻²±1.79×10⁻³、1.83×10⁻²±2.55×10⁻³和2.25×10⁻²±1.88×10⁻³微克/毫升。本研究表明，含非洲豇豆的培养基可用于生产苏云金芽孢杆菌以色列亚种原粉。
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