Poopathi Subbiah, Kumar K Anup
Centre for Research in Medical Entomology (ICMR), 4 Sarojini Street, Chinna Chokkikulam, Madurai 625002, Tamil Nadu, India.
J Econ Entomol. 2003 Aug;96(4):1039-44. doi: 10.1093/jee/96.4.1039.
The production of Bacillus thuringiensis subsp. israelensis (deBarjac) (Bti) as a biopesticide is not cost-effective using existing fermentation technology. In this study, we explored the use of several less expensive alternative culture media (potato, common sugar, and Bengal gram) for the growth and production of Bti. Growth was obtained in all tested media and was comparable to that obtained in conventional medium (Luria-Bertani). Toxicity assays showed that the toxin produced from the novel growth media were effective in killing larvae of Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti and toxicity was comparable to that produced from Luria-Bertani medium. These observations suggest that potato can be used as a cheap source of culture medium for the production of Bti toxin in mosquito control programs.
利用现有的发酵技术生产苏云金芽孢杆菌以色列亚种（deBarjac）（Bti）作为生物杀虫剂并不具有成本效益。在本研究中，我们探索了使用几种成本较低的替代培养基（土豆、普通糖和鹰嘴豆）来培养Bti并使其产生。在所有测试培养基中均能实现生长，且与在传统培养基（Luria-Bertani培养基）中获得的生长情况相当。毒性试验表明，新型生长培养基产生的毒素对致倦库蚊、斯氏按蚊和埃及伊蚊的幼虫具有杀灭效果，且毒性与Luria-Bertani培养基产生的毒素相当。这些观察结果表明，在蚊虫控制项目中，土豆可作为生产Bti毒素的廉价培养基来源。
