Akberali H B, Earnshaw M J, Marriott K R
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;77(2):289-94. doi: 10.1016/0742-8413(84)90015-x.
The direct addition of Cu2+ to unfertilized eggs of Mytilus edulis results in a stimulation of respiration with maximal stimulation occurring at a Cu2+ concentration of ca 0.5 mM. By contrast, the addition of Zn2+ has no effect on egg respiration. The uncoupler CCCP produces a 5/6 fold stimulation of egg respiration but the addition of ADP leads to only a small release of respiration. In contrast, sperm respiration is unaffected by Cu2+, inhibited by Zn2+ and CCCP produces only a small respiratory stimulation. The addition of Cu2+ to respiring Mytilus mantle tissue mitochondria produces an initial stimulation of State 4 oxidation which is then followed by a progressive inhibition. It is suggested that respiration in the unfertilized egg may be inhibited by a high ATP/ADP ratio in the cytosol. Respiration can, therefore, be released by either the addition of a H+-translocating uncoupler or by Cu2+ which may act by stimulating mitochondrial K+ influx.
向紫贻贝未受精卵直接添加Cu2+会刺激呼吸作用，在Cu2+浓度约为0.5 mM时刺激作用达到最大。相比之下，添加Zn2+对卵呼吸没有影响。解偶联剂CCCP可使卵呼吸作用增强5/6倍，但添加ADP只会导致呼吸作用少量释放。相反，精子呼吸不受Cu2+影响，受Zn2+抑制，CCCP仅产生少量呼吸刺激。向呼吸的紫贻贝外套膜组织线粒体添加Cu2+会首先刺激状态4氧化，随后逐渐抑制。有人提出，未受精卵中的呼吸作用可能受到细胞质中高ATP/ADP比值的抑制。因此，添加H+转运解偶联剂或Cu2+均可释放呼吸作用，Cu2+可能通过刺激线粒体K+内流起作用。
