Holcomb M, Cloud J G, Woolsey J, Ingermann R L
Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Box 443051, Moscow, ID 83844-3051, USA.
Comp Biochem Physiol A Mol Integr Physiol. 2004 Jul;138(3):349-54. doi: 10.1016/j.cbpb.2004.05.007.
A negative correlation between oxygen consumption and fertility was observed in both steelhead and chinook salmon eggs. However, this relationship was attributed to bacterial growth. Elimination of samples with bacterial growth resulted in no significant relationship between the rate of oxygen consumption (VO2) and fertility. VO2 of unfertilized eggs of both steelhead and chinook salmon was measured over a storage period of up to 24 days (d). Despite declines in fertility during storage, VO2 did not significantly change throughout storage. The average respiration rate for steelhead eggs was 3.4 nmol O2 per egg per h, and was 4.3 nmol O2 per egg per h for chinook salmon eggs. Treatment of chinook salmon eggs with uncouplers of mitochondrial respiration, 2,4-dinitrophenol (2,4-DNP) and carbonyl cyanide 4-trifluoro-methoxyphenylhydrazone (FCCP), resulted in an increase in VO2 to 12.9 and 11.5 nmol O2 per egg per h, respectively. Treatment with the putative uncoupler, clove oil, resulted in no change in VO2, while KCN, an inhibitor of oxidative phosphorylation, reduced oxygen consumption to zero. Copper caused an increase in oxygen consumption, even in the absence of eggs, suggesting a need for caution in interpreting changes in respiration rates as a result of metal exposure. Thus, unfertilized salmonid eggs demonstrated submaximal VO2, which was not correlated with fertility.
在虹鳟和奇努克鲑鱼的鱼卵中均观察到氧气消耗与繁殖力之间呈负相关。然而，这种关系归因于细菌生长。去除有细菌生长的样本后，氧气消耗率（VO₂）与繁殖力之间不存在显著关系。在长达24天的储存期内，对虹鳟和奇努克鲑鱼未受精卵的VO₂进行了测量。尽管储存期间繁殖力下降，但整个储存过程中VO₂并未显著变化。虹鳟鱼卵的平均呼吸速率为每枚卵每小时3.4纳摩尔氧气，奇努克鲑鱼卵为每枚卵每小时4.3纳摩尔氧气。用线粒体呼吸解偶联剂2,4-二硝基苯酚（2,4-DNP）和羰基氰化物4-三氟甲氧基苯腙（FCCP）处理奇努克鲑鱼卵，导致VO₂分别增加到每枚卵每小时12.9和11.5纳摩尔氧气。用假定的解偶联剂丁香油处理，VO₂没有变化，而氧化磷酸化抑制剂氰化钾将氧气消耗降至零。即使没有鱼卵，铜也会导致氧气消耗增加，这表明在将呼吸速率变化解释为金属暴露的结果时需要谨慎。因此，未受精的鲑科鱼卵表现出次最大VO₂，且与繁殖力无关。
