Sasaki T, Asano T, Takakura K, Sano K, Kassell N F
J Neurosurg. 1984 Jun;60(6):1186-91. doi: 10.3171/jns.1984.60.6.1186.
The purpose of this experiment was to study the nature of vasoactive substance in cerebrospinal fluid (CSF) from patients with aneurysmal subarachnoid hemorrhage. The authors have examined the effects of disulfide bond-reducing agents, a sulfhydryl group oxidizing agent, and specific antagonists of so-called "classical" neurotransmitters on the in vitro isometric contraction of canine basilar arterial strips induced by bloody human CSF. The disulfide bond-reducing agents dithiothreitol (10(-4) M) and dithioerythritol (10(-4) M) suppressed the contraction induced by bloody CSF by an average of 40.3% and 61.2%, respectively. This suppression was achieved under conditions that did not alter KCl-induced contraction. The sulfhydryl group oxidizing agent, 5,5'-dithiobis-(2-nitrobenzoic acid), 10(-4) M, reversed the inhibitory effect of dithioerythritol on the contractile response to bloody CSF. No significant suppression of any response in any preparation was observed with methysergide (10(-7) M), mepyramine (10(-7) M), phenoxybenzamine (10(-5) M), propranolol (10(-6) M), or atropine (10(-6) M). These results indicate that disulfide bonds in the arterial smooth-muscle cells are involved in the contractile responses of canine basilar artery to bloody CSF. Prostaglandins, hemoglobin, and lipid hydroperoxides may all be spasmogens in bloody CSF, while serotonin, histamine, norepinephrine, and acetylcholine are probably not involved.
本实验的目的是研究动脉瘤性蛛网膜下腔出血患者脑脊液（CSF）中血管活性物质的性质。作者检测了二硫键还原剂、巯基氧化剂以及所谓“经典”神经递质的特异性拮抗剂对人血性脑脊液诱导的犬基底动脉条体外等长收缩的影响。二硫键还原剂二硫苏糖醇（10⁻⁴M）和二硫赤藓糖醇（10⁻⁴M）分别平均抑制血性脑脊液诱导的收缩40.3%和61.2%。这种抑制是在不改变氯化钾诱导收缩的条件下实现的。巯基氧化剂5,5'-二硫双（2-硝基苯甲酸）（10⁻⁴M）逆转了二硫赤藓糖醇对血性脑脊液收缩反应的抑制作用。麦角新碱（10⁻⁷M）、美吡拉敏（10⁻⁷M）、酚苄明（10⁻⁵M）、普萘洛尔（10⁻⁶M）或阿托品（10⁻⁶M）对任何制剂中的任何反应均无显著抑制作用。这些结果表明，动脉平滑肌细胞中的二硫键参与了犬基底动脉对血性脑脊液的收缩反应。前列腺素、血红蛋白和脂质氢过氧化物可能都是血性脑脊液中的致痉物质，而血清素、组胺、去甲肾上腺素和乙酰胆碱可能不参与其中。
