Stuckhardt J L, Poppe S M
Teratog Carcinog Mutagen. 1984;4(2):181-8. doi: 10.1002/tcm.1770040203.
A method for examining the viscera of rat and rabbit fetuses is described. Techniques used in detecting visceral alterations in rats and rabbits for routine teratogenicity screens have varied over the years. The method used should be quick and simple but at the same time must be accurate, reliable, and comprehensive. The procedure used in this lab is a complete and systematic necropsy of the fresh fetus requiring minimum equipment and time. The examination can be done immediately following cesarean section and yields an intact skeleton which can subsequently be processed for skeletal examination. The fresh specimen and the natural coloration of in situ organs makes color photography of visceral alterations clear and concise. Any lesion can be appropriately fixed for histopathic examination. This technique begins with the examination of the organs in the abdominal cavity and proceeds to the thorax. Of special interest is the procedure used to inspect the internal anatomy of the fresh fetal heart. A description of the internal examination of both rat and rabbit heads and eyes is also included.
本文描述了一种检查大鼠和兔胎儿内脏的方法。多年来，用于常规致畸性筛查中检测大鼠和兔内脏改变的技术各不相同。所使用的方法应快速简便，但同时必须准确、可靠且全面。本实验室采用的程序是对新鲜胎儿进行完整而系统的尸检，所需设备和时间最少。检查可在剖宫产术后立即进行，并能得到完整的骨骼，随后可对其进行骨骼检查。新鲜标本和原位器官的自然色泽使内脏改变的彩色照片清晰明了。任何病变都可适当固定以便进行组织病理学检查。该技术首先检查腹腔内的器官，然后检查胸部。特别值得关注的是用于检查新鲜胎儿心脏内部结构的程序。本文还包括对大鼠和兔头部及眼睛内部检查的描述。
