Ishida M, Shinozaki H
Br J Pharmacol. 1984 Jun;82(2):523-31. doi: 10.1111/j.1476-5381.1984.tb10789.x.
The effect of some alkaloids from Sophora flavescens (matrine, oxymatrine and N-methylcytisine) on glutamate-induced responses was investigated using electrophysiological techniques at the crayfish neuromuscular junction. At concentrations greater than 0.1 mM, matrine depressed both glutamate-induced responses and neurally evoked excitatory junctional potentials. Oxymatrine was less powerful than matrine, and N-methylcytisine was not effective. Matrine also depressed quisqualate-induced responses at this site, but did not have an effect on gamma-aminobutyric acid (GABA)-induced responses. Matrine had no influence upon either the resting membrane potential or the input resistance of the crayfish opener muscle. The inhibition of the glutamate-induced response by matrine was not affected by the membrane potential of the muscle fibre. Matrine reduced the size of extracellularly recorded excitatory junctional potentials without affecting their quantal content. Matrine did not affect the decay of extracellular excitatory junctional currents at the resting membrane potential. The results presented clearly demonstrate that matrine has an inhibitory action on glutamate-induced responses.
利用电生理技术，在小龙虾神经肌肉接头处研究了苦参中的一些生物碱（苦参碱、氧化苦参碱和N-甲基野靛碱）对谷氨酸诱导反应的影响。浓度大于0.1 mM时，苦参碱会抑制谷氨酸诱导的反应以及神经诱发的兴奋性接头电位。氧化苦参碱的作用比苦参碱弱，而N-甲基野靛碱无效。苦参碱在此部位也会抑制 quisqualate 诱导的反应，但对γ-氨基丁酸（GABA）诱导的反应没有影响。苦参碱对小龙虾 opener 肌肉的静息膜电位或输入电阻均无影响。苦参碱对谷氨酸诱导反应的抑制作用不受肌肉纤维膜电位的影响。苦参碱减小了细胞外记录的兴奋性接头电位的大小，但不影响其量子含量。苦参碱在静息膜电位时不影响细胞外兴奋性接头电流的衰减。所呈现的结果清楚地表明，苦参碱对谷氨酸诱导的反应具有抑制作用。
