在以谷氨酸作为碳源和氮源生长的深红红螺菌培养物中添加苹果酸后固氮酶的去阻遏作用。
Derepression of nitrogenase by addition of malate to cultures of Rhodospirillum rubrum grown with glutamate as the carbon and nitrogen source.
作者信息
Hoover T R, Ludden P W
出版信息
J Bacteriol. 1984 Jul;159(1):400-3. doi: 10.1128/jb.159.1.400-403.1984.
Abstract
Rhodospirillum rubrum grown in continuous culture with glutamate as the sole fixed C and N source produced no nitrogenase, and the cultures were characterized by high extracellular ammonium concentrations. Addition of organic acids derepressed nitrogenase. Glutamate dehydrogenase, glutamine synthetase, glutamate synthase, malate dehydrogenase, nitrogenase, and ammonium were assayed before and after malate addition.
摘要
在以谷氨酸作为唯一固定碳源和氮源的连续培养中生长的深红红螺菌不产生固氮酶，且培养物的特征是细胞外铵浓度很高。添加有机酸可解除对固氮酶的阻遏。在添加苹果酸之前和之后测定了谷氨酸脱氢酶、谷氨酰胺合成酶、谷氨酸合酶、苹果酸脱氢酶、固氮酶和铵的含量。
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