Towle A C, Lauder J M, Joh T H
J Histochem Cytochem. 1984 Jul;32(7):766-70. doi: 10.1177/32.7.6145741.
Immunocytochemical localization of tyrosine hydroxylase (TH) was performed on paraffin sections pretreated with various proteolytic enzymes. It was found that pretreatment with trypsin (1.2 mg/ml) for 5 min resulted in a dramatic increase in the number of TH-positive terminals throughout the brain, especially in the cerebellum, which contains fine preterminal and terminal axons that are difficult to stain. This pretreatment also led to a significant reduction in background staining and allowed for the use of the TH antiserum at high working dilutions. Several other proteolytic enzymes were tested and only chymotrypsin was nearly as effective as trypsin with respect to TH staining.
在经各种蛋白水解酶预处理的石蜡切片上进行酪氨酸羟化酶（TH）的免疫细胞化学定位。发现用胰蛋白酶（1.2mg/ml）预处理5分钟会导致整个大脑中TH阳性终末数量急剧增加，尤其是在小脑，小脑中含有难以染色的细小终末前和终末轴突。这种预处理还导致背景染色显著减少，并允许在高工作稀释度下使用TH抗血清。还测试了其他几种蛋白水解酶，就TH染色而言，只有胰凝乳蛋白酶几乎与胰蛋白酶一样有效。
