DeAngelo A B, Garrett C T, Queral A E
Cancer Lett. 1984 Jul;23(3):323-30. doi: 10.1016/0304-3835(84)90100-9.
The effect of di(2-ethylhexyl)phthalate (DEHP), a widely used plasticizer and environmental contaminant, on the emergence of gamma-glutamyltranspeptidase positive (GGT+) preneoplastic foci in the liver of rats fed promoting diets was studied. GGT+ foci were initiated in the liver of Sprague--Dawley male rats with a single dose of diethylnitrosamine (DEN) following partial hepatectomy. One series of control rats received saline vehicle alone. Promotion of foci was commenced by feeding: (1) a choline-deficient diet (CD); (2) a choline-supplemented diet (CS) containing 0.06% phenobarbital (CS + PHB); or (3) a CD diet containing 0.06% phenobarbital (CD + PHB). In the absence of initiation by DEN, dietary treatments did not increase the number of GGT+ foci. In rats receiving DEN, each promoting regimen effectively increased the number of GGT+ foci above levels in control rats fed only the choline-supplemented diet. Inclusion of the plasticizer at a level of 2% in each of the dietary promotion treatments, however, effectively inhibited the appearance of the foci.
研究了广泛使用的增塑剂和环境污染物邻苯二甲酸二（2-乙基己基）酯（DEHP）对喂食促癌饮食的大鼠肝脏中γ-谷氨酰转肽酶阳性（GGT+）癌前病灶出现的影响。在部分肝切除术后，用单剂量二乙基亚硝胺（DEN）在斯普拉格-道利雄性大鼠肝脏中引发GGT+病灶。一组对照大鼠仅接受生理盐水载体。通过喂食开始促进病灶发展：（1）胆碱缺乏饮食（CD）；（2）含有0.06%苯巴比妥的胆碱补充饮食（CS+PHB）；或（3）含有0.06%苯巴比妥的CD饮食（CD+PHB）。在没有DEN引发的情况下，饮食处理不会增加GGT+病灶的数量。在接受DEN的大鼠中，每种促癌方案都有效地使GGT+病灶的数量增加到仅喂食胆碱补充饮食的对照大鼠的水平之上。然而，在每种饮食促癌处理中加入2%水平的增塑剂，有效地抑制了病灶的出现。
