黄斑蝾螈（Ambystoma maculatum）肝脏中镉、铜和锌的分布及其与金属硫蛋白的结合

Distribution of cadmium, copper and zinc in the liver of spot salamander, Ambystoma maculatum and their binding to metallothionein.

作者信息

Suzuki K T, Ebihara Y

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):35-8. doi: 10.1016/0742-8413(84)90043-4.

Abstract

Cadmium (Cd) was injected intramuscularly into male spot salamanders, Ambystoma maculatum. Concentrations and contents of Cd, zinc (Zn), copper (Cu), iron, magnesium and calcium in the livers of control and Cd-injected animals were determined simultaneously. A low molecular weight Cd, Cu, Zn-binding protein present in the hepatic supernatant prepared from control and Cd-injected animals was characterized as metallothionein (MT) from several properties characteristic of MT. MT induced and present in the liver supernatants of spot salamanders consisted of a single isoform.

摘要

将镉（Cd）肌肉注射到雄性黄斑蝾螈（Ambystoma maculatum）体内。同时测定了对照动物和注射镉的动物肝脏中镉、锌（Zn）、铜（Cu）、铁、镁和钙的浓度及含量。从对照动物和注射镉的动物制备的肝脏上清液中存在的一种低分子量镉、铜、锌结合蛋白，根据金属硫蛋白（MT）的几个特性被鉴定为MT。黄斑蝾螈肝脏上清液中诱导产生并存在的MT由单一异构体组成。