激素和环磷酸腺苷对大鼠乳腺外植体γ-谷氨酰转肽酶活性的影响。
Effect of hormones and cyclic AMP on gamma-glutamyltranspeptidase activity of rat mammary gland explants.
作者信息
Valdivieso M P, Puente J F, Sapag-Hagar M
出版信息
Experientia. 1984 Jul 15;40(7):710-1. doi: 10.1007/BF01949736.
gamma-Glutamyl transpeptidase activity was assayed in midpregnant rat mammary gland explants at 14, 22 and 38 h, in the presence and absence of insulin, prolactin and corticosterone. With these 3 hormones the explants attained the characteristics of a secretory gland after 22 h of tissue culture, at which time the enzyme exhibited its maximal activity. The addition of dibutyryl cyclic AMP in the presence of the 3 hormones produced a significant increase in enzyme activity, which was maximal with a 1 mM concentration of the cyclic nucleotide. A similar effect was observed when theophylline or theophylline plus dibutyryl cyclic AMP were added to the culture medium.
在怀孕中期大鼠乳腺外植体培养14、22和38小时后，分别在有和没有胰岛素、催乳素及皮质酮的情况下测定γ-谷氨酰转肽酶活性。在这三种激素作用下，外植体在组织培养22小时后具备了分泌腺的特征，此时该酶表现出最大活性。在这三种激素存在的情况下添加二丁酰环磷腺苷，可使酶活性显著增加，在环核苷酸浓度为1 mM时达到最大值。当向培养基中添加茶碱或茶碱加二丁酰环磷腺苷时，也观察到类似效果。
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