一种用于记录骨骼肌单通道电流的新制剂。
A new preparation for recording single-channel currents from skeletal muscle.
作者信息
Standen N B, Stanfield P R, Ward T A, Wilson S W
出版信息
Proc R Soc Lond B Biol Sci. 1984 Jun 22;221(1225):455-64. doi: 10.1098/rspb.1984.0044.
PMID:6146986
Abstract
Spherical vesicles of sarcolemma (media diameter, 78 microns) were prepared from frog skeletal muscle by prolonged exposure to enzymes (collagenase, then protease) and to isotonic KCl solutions, which promote swelling of muscle fibres. Patch clamp recording was used to measure unitary K and Na currents from this preparation. Na and K channels had conductances around 15 pS and their kinetic properties appeared unaltered by vesicle formation.
摘要
通过长时间暴露于酶（胶原酶，然后是蛋白酶）和等渗氯化钾溶液中，从青蛙骨骼肌制备了肌膜球形囊泡（平均直径78微米），这些溶液会促使肌肉纤维肿胀。使用膜片钳记录来测量该制剂中的单位钾电流和钠电流。钠通道和钾通道的电导约为15皮西门子，并且它们的动力学特性似乎未因囊泡形成而改变。
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