Bogatskiĭ A V, Golovenko N Ia, Meteshkin Iu V, Bukin V A
Biull Eksp Biol Med. 1984 Aug;98(8):196-8.
Phenazepam (I) and 3-hydroxymetabolite (II) interacting with cytochrome P-450 of the liver of rats administered phenobarbital and 3-methylcholantrene demonstrated the 2nd type of spectral changes in hemoprotein. The binding constants of I and II considerably differ, which points to the possibility of interaction of substrates with different areas of hemoproteins. The characteristic points (maximum, minimum, isobestic) of the cytochrome P-450 spectrum were displaced during II titration to the longwave region as compared with I. The calculation of electron density on the atoms of the heterocycle and aromatic nuclei of the molecules of I and II has shown that the differences in the spectra of I and II binding with cytochrome P-450 are determined by the distribution of electron density of their heterocycle.
与经苯巴比妥和3-甲基胆蒽处理的大鼠肝脏细胞色素P-450相互作用的芬那西泮（I）和3-羟基代谢物（II）表现出血红蛋白的第二种光谱变化类型。I和II的结合常数差异很大，这表明底物可能与血红蛋白的不同区域相互作用。与I相比，在II滴定过程中细胞色素P-450光谱的特征点（最大值、最小值、等吸收点）向长波区域移动。对I和II分子杂环和芳环原子核上原子的电子密度计算表明，I和II与细胞色素P-450结合光谱的差异是由其杂环电子密度分布决定的。
