Virji M, Heckels J E
J Gen Microbiol. 1984 May;130(5):1089-95. doi: 10.1099/00221287-130-5-1089.
A panel of monoclonal antibodies with varying pilus specificities ranging from cross-reacting to type-specific has been used to investigate the role of conserved and variable antigenic domains in the adhesion of gonococci to human epithelial cells. The binding of 125I-labelled alpha pili from strain P9 to buccal epithelial cells was inhibited by three type-specific but not by two cross-reacting antibodies. Four type-specific antibodies inhibited the binding of gamma pili while the two cross-reacting antibodies were again without effect. The virulence of the variants P9-2 (alpha pili) and P9-35 (gamma pili) for Chang conjunctiva epithelial cells was similarly reduced only in the presence of relevant type-specific antibodies. These results indicate the importance of variable antigenic domains in the adhesion of gonococci to human epithelial cells.
一组具有不同菌毛特异性（从交叉反应到型特异性）的单克隆抗体已被用于研究保守和可变抗原结构域在淋球菌黏附人上皮细胞中的作用。来自菌株P9的125I标记的α菌毛与颊上皮细胞的结合被三种型特异性抗体抑制，但未被两种交叉反应抗体抑制。四种型特异性抗体抑制了γ菌毛的结合，而两种交叉反应抗体再次无效。变体P9-2（α菌毛）和P9-35（γ菌毛）对张氏结膜上皮细胞的毒力仅在存在相关型特异性抗体时同样降低。这些结果表明可变抗原结构域在淋球菌黏附人上皮细胞中的重要性。
