Yamamoto T, Yamamoto K, Kawasaki K, Yaoita E, Shimizu F, Kihara I
Nephron. 1986;43(4):293-8. doi: 10.1159/000183857.
Mesangial cells of F344 rats degenerated and then disappeared within 2 days after the intravenous administration of rabbit antirat thymocyte serum (ATS). Rabbit IgG and rat C3 were identified in the mesangium in the rat glomeruli. To establish the glomerular binding site of ATS administered intravenously into rats, one kidney of each rat given ATS intravenously 12 h earlier was perfused ex vivo through the renal artery with peroxidase-labeled antirabbit IgG followed by sequential glutaraldehyde and diaminobenzidine perfusions to minimize the ultrastructural damage. The other kidney was removed before the perfusion for histologic study to examine the glomerular injury. The rabbit IgG identified by peroxidase-reaction product was present diffusely in the glomerular mesangium when viewed by light microscopy and exclusively on the surfaces of most mesangial cells by electron microscopy. Immunofluorescence microscopy showed rabbit IgG essentially in the mesangium, and electron microscopy revealed the degeneration of mesangial cells in the kidneys that had been removed before the surgical perfusion. However, no histological abnormalities were found in the kidneys from control rats given ATS absorbed with rat thymocytes. The present study showed that the intravenous administration of ATS into rats induced the extensive mesangial cell damage by the binding of ATS to Thy-1 antigens on the mesangial cells.
在给F344大鼠静脉注射兔抗大鼠胸腺细胞血清（ATS）后2天内，大鼠的系膜细胞发生退变，然后消失。在大鼠肾小球的系膜区鉴定出兔IgG和大鼠C3。为了确定静脉注射给大鼠的ATS在肾小球的结合位点，对每只在12小时前静脉注射了ATS的大鼠，通过肾动脉对其中一个肾脏进行离体灌注，灌注液为过氧化物酶标记的抗兔IgG，随后依次进行戊二醛和二氨基联苯胺灌注，以尽量减少超微结构损伤。另一个肾脏在灌注前取出用于组织学研究，以检查肾小球损伤情况。通过光学显微镜观察，经过氧化物酶反应产物鉴定的兔IgG弥漫性地存在于肾小球系膜区，而通过电子显微镜观察，其仅存在于大多数系膜细胞的表面。免疫荧光显微镜检查显示兔IgG主要存在于系膜区，电子显微镜检查显示在手术灌注前取出的肾脏中系膜细胞发生退变。然而，给用大鼠胸腺细胞吸收过的ATS的对照大鼠的肾脏未发现组织学异常。本研究表明，给大鼠静脉注射ATS会通过ATS与系膜细胞上的Thy-1抗原结合，导致广泛的系膜细胞损伤。
