Immunocytochemistry of luteinizing hormone-releasing hormone in brains of breeding eastern narrow-mouthed toads (Gastrophryne carolinensis).

Unlabeled antibody-enzyme immunocytochemistry was used to localize mammalian-like immunoreactive luteinizing hormone-releasing hormone in brains of breeding eastern narrow-mouthed toads (Gastrophryne carolinensis). Intense immunostaining occurred over the infundibular floor and the median eminence, yet staining of few perikarya in the anterior preoptic area was minimal. This pattern of markedly regional immunostaining differs from that reported for other anurans.