Juprelle-Soret M, Wattiaux-De Coninck S, Wattiaux R
Eur J Cell Biol. 1984 Jul;34(2):271-4.
The intracellular distribution of rat liver transglutaminase has been investigated by centrifugation methods. When measured in presence of Ca++ the enzyme is mainly present in the unsedimentable fraction of the homogenate. When assayed in absence of Ca++, the enzymatic activity exhibits a distribution pattern like that of lysosomal markers, both after differential and isopycnic centrifugation. The enzyme shows the phenomenom of structure linked latency and can be unmasked parallel with acid phosphatase by freezing and thawing. The origin of this transglutaminase is discussed; it is proposed that it is a genuine constituent of lysosomes.
通过离心方法研究了大鼠肝脏转谷氨酰胺酶的细胞内分布。当在Ca++存在的情况下进行测定时，该酶主要存在于匀浆的不可沉降部分。在没有Ca++的情况下进行测定时，无论是在差速离心还是等密度离心后，酶活性都呈现出与溶酶体标记物相似的分布模式。该酶表现出结构相关的潜伏现象，并且可以通过冻融与酸性磷酸酶同时被揭示出来。讨论了这种转谷氨酰胺酶的来源；有人提出它是溶酶体的一种真正成分。
