Gotoh M, Miyake K, Mitsuya H
Urol Res. 1984;12(3):159-64. doi: 10.1007/BF00255915.
Leydig cell number was evaluated quantitatively in testicular biopsies from post-pubertal cryptorchid patients and normal controls. For this quantitative evaluation we used the following method. This is based on the determination of the total number of Leydig cells, Leydig cell clusters and seminiferous tubules in the entire histologic sections of each biopsy and the determination of the following indices; mean Leydig cells per tubule, mean Leydig cell clusters per tubule and mean Leydig cells per cluster. In addition, the numbers of Sertoli cells were counted, and Leydig-Sertoli cell ratio was also determined. These indices were correlated with each other. All indices were significantly elevated not only in undescended but in contralateral scrotal testes of the cryptorchid patients in comparison to those in normal controls. Between undescended and descended scrotal testes of the same individual patients, those indices were significantly higher in the descended scrotal testes than in the undescended ones. Thus, Leydig cell hyperplasia was noted in the testes of post-pubertal cryptorchid patients, and was more prominent in the contralateral scrotal testes than in the undescended ones.
对青春期后隐睾患者和正常对照者的睾丸活检组织进行了Leydig细胞数量的定量评估。为此定量评估，我们采用了以下方法。该方法基于对每个活检组织的整个组织学切片中Leydig细胞总数、Leydig细胞簇和生精小管的测定，以及以下指标的测定：每根小管的平均Leydig细胞数、每根小管的平均Leydig细胞簇数和每个细胞簇的平均Leydig细胞数。此外，还对支持细胞数量进行了计数，并确定了Leydig细胞与支持细胞的比例。这些指标相互关联。与正常对照相比，隐睾患者未降睾丸和对侧阴囊睾丸中的所有指标均显著升高。在同一患者的未降睾丸和已降阴囊睾丸之间，已降阴囊睾丸中的这些指标显著高于未降睾丸。因此，青春期后隐睾患者的睾丸中存在Leydig细胞增生，且对侧阴囊睾丸中的增生比未降睾丸更为明显。
