Lechan R M, Lin H D, Ling N, Jackson I M, Jacobson S, Reichlin S
Brain Res. 1984 Aug 20;309(1):55-61. doi: 10.1016/0006-8993(84)91010-2.
Using an antiserum which reacts with the carboxyl terminus of GRF(1-44)NH2, the distribution of immunoreactive growth hormone releasing factor (GRF) in the rhesus monkey hypothalamus was delineated by peroxidase immunocytochemistry. Immunoreactive material was present in dense terminal fields in the median eminence closely associated with portal capillaries but in a location distinct from that noted for immunoreactive thyrotropin-releasing hormone (TRH) or somatostatin. GRF-immunoreactive cell bodies were identified in the arcuate nucleus and ventromedial nucleus. These studies provide evidence for the presence of GRF(1-44)NH2 in the primate brain and demonstrate that in the hypothalamus it is localized exclusively in cells and fibers corresponding to the tuberoinfundibular system.
使用一种能与生长激素释放因子（GRF）（1 - 44）NH₂ 的羧基末端发生反应的抗血清，通过过氧化物酶免疫细胞化学法描绘了恒河猴下丘脑内免疫反应性生长激素释放因子（GRF）的分布情况。免疫反应性物质存在于正中隆起处与门静脉毛细血管紧密相关的密集终末区域，但所处位置与免疫反应性促甲状腺激素释放激素（TRH）或生长抑素不同。在弓状核和腹内侧核中鉴定出了GRF免疫反应性细胞体。这些研究为灵长类动物大脑中存在GRF（1 - 44）NH₂ 提供了证据，并表明在下丘脑中它仅定位于与结节漏斗系统相对应的细胞和纤维中。
