Weber C, Schmid V
Exp Cell Res. 1984 Nov;155(1):153-62. doi: 10.1016/0014-4827(84)90776-6.
In vivo gap junctions (gj) are common in the subumbrellar plate endoderm of anthomedusa. When isolated and cultivated in artificial sea water the tissue, consisting of one cell type only, forms a spheroid in which all gap junctions disappear. Gap junction (gj) formation can, however, be induced by attachment and consecutive spreading of the endodermal tissue (spheroid) on stretched extracellular matrix (ECM) material isolated from the polyp stage (with Ca2+-Mg2+-free sea water, without EDTA). Formation, and loss of gj is reversible and strictly corresponds with the alteration from the monolayer 'spread' (on stretched ECM) to 'spheroid' arrangement (no ECM) of the endodermal cells. The functional competence of induced gj is ascertained by injection of Lucifer Yellow, and the transfer of the dye is used to map the pattern of communication. The experimental conditions that result in gj formation simulate the in vivo situation of the endoderm. The influence of the ECM on gj formation, and the structural organization of the isolated endodermal tissue in this well defined in vitro system are discussed.
体内间隙连接（gj）在花水母亚伞板内胚层中很常见。当分离并在人工海水中培养时，仅由一种细胞类型组成的组织会形成一个球体，其中所有间隙连接都会消失。然而，间隙连接（gj）的形成可以通过内胚层组织（球体）附着并连续铺展在从水螅体阶段分离的拉伸细胞外基质（ECM）材料上（用无钙镁海水，不含乙二胺四乙酸）来诱导。gj的形成和丧失是可逆的，并且与内胚层细胞从单层“铺展”（在拉伸的ECM上）到“球体”排列（无ECM）的变化严格对应。通过注射荧光黄来确定诱导的gj的功能能力，并使用染料转移来绘制通讯模式。导致gj形成的实验条件模拟了内胚层的体内情况。讨论了ECM对gj形成的影响以及在这个定义明确的体外系统中分离的内胚层组织的结构组织。
