Sarmiento M, Loken M R, Fitch F W
Hybridoma. 1981;1(1):13-26. doi: 10.1089/hyb.1.1981.1.13.
The molecule bearing the Thy-1.2 antigen, T25, has been isolated from fractionated thymocytes as well as from cloned cytolytic and helper T cells by immune precipitation with monoclonal antibodies. Using NaDodSO4/polyacrylamide gels cross-linked with N,N'-diallytartardiamide, electrophoretic analyses of the precipitates revealed a heterogeneous pattern of polypeptide bands, ranging in apparent molecular weight from 28,000-36,000 daltons. Thymocytes separated on the basis of Thy-1.2 surface antigen density were found to differ not only in amount of Thy-1.2 expressed, but also in the structure of the surface T25 molecules isolated from each subpopulation. This structural variation was evidenced in changes detected in the relative density and electrophoretic mobility of T25 band patterns obtained on SDS gels. Differences in T25 band patterns were also observed in polypeptides isolated from cloned cytolytic or helper T cells. These data suggest that although surface T25 is structurally heterogeneous, the pattern of heterogeneity can differ from cell to cell, or from population to population. Changes in T25 structure appear to correlated with differences in either T lymphocyte maturation or T cell function.
携带Thy-1.2抗原的分子T25，已通过用单克隆抗体进行免疫沉淀，从分级分离的胸腺细胞以及克隆的溶细胞性T细胞和辅助性T细胞中分离出来。使用与N，N'-二烯丙基酒石酸二酰胺交联的十二烷基硫酸钠/聚丙烯酰胺凝胶，对沉淀物进行的电泳分析显示出多肽条带的异质性模式，其表观分子量范围为28,000 - 36,000道尔顿。基于Thy-1.2表面抗原密度分离的胸腺细胞不仅在Thy-1.2表达量上存在差异，而且从每个亚群分离出的表面T25分子的结构也有所不同。这种结构变异在SDS凝胶上获得的T25条带模式的相对密度和电泳迁移率的变化中得到证实。从克隆的溶细胞性T细胞或辅助性T细胞中分离出的多肽中也观察到T25条带模式的差异。这些数据表明，尽管表面T25在结构上是异质的，但异质性模式可能因细胞而异，或因群体而异。T25结构的变化似乎与T淋巴细胞成熟或T细胞功能的差异相关。
