大鼠组织中苄胺氧化酶活性的年龄相关性变化。

Age-related changes in benzylamine oxidase activity in rat tissues.

作者信息

Cao Danh H, Strolin Benedetti M, Dostert P, Mousset A

出版信息

J Pharm Pharmacol. 1984 Sep;36(9):592-6. doi: 10.1111/j.2042-7158.1984.tb04904.x.

DOI:10.1111/j.2042-7158.1984.tb04904.x

Abstract

Brain, liver, heart, lung, kidney and duodenum benzylamine oxidase (BZAO) activities were measured from young and old rats. Protein content was found to decrease in liver (-17%), kidney (-20%) and duodenum (-17%) but remained unchanged in brain, heart and lung of old rats compared with that of young rats. A significant decrease (-41%) of BZAO activity was found in lung whereas a significant increase of enzyme activity was found in brain (+49%) and kidney (+25%) and no change was found in heart and duodenum of old rats. BZAO was not detected in either young or old rat liver. Kinetic analysis for lung BZAO activity of old rats showed that Vmax was decreased but Km was unchanged in comparison with that of young rats. Since, as we have shown previously, MAO-A and -B activity in lung of old rats was also found to be decreased, the decrease of lung BZAO activity with increasing age merits further investigation, lung playing an important role in removing amines from the circulation.

摘要

测定了年轻和老年大鼠脑、肝、心、肺、肾及十二指肠中苄胺氧化酶（BZAO）的活性。结果发现，老年大鼠肝脏（-17%）、肾脏（-20%）和十二指肠（-17%）中的蛋白质含量下降，而脑、心和肺中的蛋白质含量与年轻大鼠相比无变化。老年大鼠肺中BZAO活性显著降低（-41%），而脑（+49%）和肾脏（+25%）中的酶活性显著升高，心和十二指肠中未发现变化。在年轻或老年大鼠肝脏中均未检测到BZAO。老年大鼠肺BZAO活性的动力学分析表明，与年轻大鼠相比，Vmax降低，但Km不变。由于正如我们之前所表明的，老年大鼠肺中MAO-A和-B活性也被发现降低，因此随着年龄增长肺BZAO活性的降低值得进一步研究，肺在从循环中清除胺类方面起着重要作用。