Infusion of physiological saline supplemented with adenine, pyruvate and phosphate.

After controlled bleeding 20 ml/kg enriched infusion fluid containing 0.005 mol/1 adenine, 0.1 mol/1 pyruvate, 0.1 mol/1dibasic sodium phosphate and 0.15 mol/1 sodium chloride was given to 14 dogs. Three dogs received after bleeding the same infusion fluid without adenine. One dog received only dextran after blood withdrawal and served as control Before and after bleeding and 1, 4, 24, 48 and 72 hours after infusion the levels of 2,3-DPG, ATP and P50 index were determined. After infusion of enriched fluid with and without adenine the level of 2,3-DPG and the value of P50 index increased. No rise in the ATP level was observed.