2',5'-寡聚腺苷酸的木糖腺苷类似物对聚（A）特异性、降解2',5'-寡聚腺苷酸的2',3'-外切核糖核酸酶及参与聚（A）⁺mRNA代谢的其他酶的影响

Influence of the xyloadenosine analogue of 2',5'-oligoriboadenylate on poly(A)-specific, 2',5'-oligoriboadenylate degrading 2',3'-exoribonuclease and further enzymes involved in poly(A)(+)mRNA metabolism.

作者信息

Schröder H C, Gosselin G, Imbach J L, Müller W E

出版信息

Mol Biol Rep. 1984 Dec;10(2):83-9. doi: 10.1007/BF00776979.

PMID:6152011

Abstract

The homogeneous poly(A)-specific 2',3'-exoribonuclease from calf thymus gland, which cleaves both 3',5'- and 2',5'-linked oligoriboadenylates, does not degrade (xyloA2'p)2 xyloA, the xylofuranosyladenosine analogue of the 2-5A core. This oligonucleotide, which is supposed to enter intact cells rapidly, was found to possess an increased stability and an enhanced antiherpesvirus activity compared to the natural (A2'p)2A (Eppstein, D.A., Barnett, J.W., Marsh, Y.V., Gosselin, G. and Imbach, J.-L. (1983) Nature 302, 723-724). The poly(A) anabolic enzyme, poly(A) polymerase (Mn2+-dependent), from the same source, which is initiated by (A3'p)2A and its higher oligomers, does not accept 2-5A core and its xyloadenosine analogue as primer. Both oligonucleotides exert no influence on endoribonuclease IV and on the integrity of the poly(A)-ribonucleoprotein complex.

摘要

来自小牛胸腺的均一性聚腺苷酸特异性2'，3'-外切核糖核酸酶，它能切割3'，5'-和2'，5'-连接的寡聚核糖腺苷酸，但不降解(xyloA2'p)2 xyloA，即2-5A核心的木糖呋喃糖基腺苷类似物。这种寡核苷酸被认为能迅速完整地进入细胞，与天然的(A2'p)2A相比，它具有更高的稳定性和更强的抗疱疹病毒活性（Eppstein, D.A., Barnett, J.W., Marsh, Y.V., Gosselin, G.和Imbach, J.-L. (1983) Nature 302, 723-724）。来自同一来源的聚腺苷酸合成酶，即聚腺苷酸聚合酶（依赖Mn2+），由(A3'p)2A及其更高的寡聚物起始，不接受2-5A核心及其木糖腺苷类似物作为引物。这两种寡核苷酸对核糖核酸内切酶IV和聚腺苷酸核糖核蛋白复合物的完整性均无影响。