Cerasaro M, Lenzi A, Massacesi A, Dondero F
Int J Fertil. 1984;29(3):168-75.
Three procedures commonly employed in the extraction of soluble proteins from cervical mucus, for the application of the technique for the detection of sperm antibodies also in this biological fluid, were compared in order to establish which provided the highest yield. Studies were therefore carried out on 125 women with primary and secondary sterility characterized by good rheologic qualities of their mid-cycle cervical mucus and in presence of repeatedly poor PCTs, not justified by the seminal characteristics of their partners. The laboratory results and the statistical analysis have shown that as far as concerns IgG, liquefaction with bromelin and solubilization with Baker's buffer provide a better yield than ultracentrifugation; while bromelin alone has the best yield with IgA.
为确定哪种方法能获得最高产量，对从宫颈黏液中提取可溶性蛋白质的三种常用方法进行了比较，这些方法也用于该生物液体中精子抗体检测技术。因此，对125名患有原发性和继发性不育症的女性进行了研究，她们月经周期中期宫颈黏液具有良好的流变学特性，且多次出现精子穿透试验（PCT）结果不佳，但并非由其伴侣的精液特征所致。实验室结果和统计分析表明，就IgG而言，用菠萝蛋白酶液化和用贝克缓冲液溶解比超速离心能获得更好的产量；而就IgA而言，单独使用菠萝蛋白酶的产量最高。
