Leroy P, Nicolas A
J Chromatogr. 1984 Dec 28;317:513-21. doi: 10.1016/s0021-9673(01)91691-7.
To assay various secondary amino drugs (sympatomimetic, beta-blocking, antiarrhythmic agents), they were converted to metal (copper or nickel) dithiocarbamate complexes by means of a pre-column derivatization method. The electrochemical properties of the complexes were studied. They were chromatographed on a reversed-phase column (LiChrosorb RP-18) with mixtures of acetate buffer (pH 5.8) and organic solvents (methanol, acetonitrile, ethanol or dichloromethane) as mobile phases. The complexes were detected by amperometry (applied potential of +0.7 V vs. SCE) or by UV spectrophotometry. The procedure has great sensitivity (10(-12) mole for each injected compound) and good selectivity for the more substituted amino drugs.
为了测定各种仲胺类药物（拟交感神经药、β受体阻滞剂、抗心律失常药），采用柱前衍生化法将它们转化为二硫代氨基甲酸金属（铜或镍）配合物。研究了这些配合物的电化学性质。它们在反相柱（LiChrosorb RP - 18）上进行色谱分离，以醋酸盐缓冲液（pH 5.8）和有机溶剂（甲醇、乙腈、乙醇或二氯甲烷）的混合物作为流动相。通过安培法（相对于饱和甘汞电极施加 +0.7 V 的电位）或紫外分光光度法检测这些配合物。该方法具有很高的灵敏度（每种注入化合物为 10^(-12) 摩尔），并且对取代程度更高的氨基药物具有良好的选择性。
