Misztal G, Przyborowski L
Department of Medicinal Chemistry, Medical Academy in Lublin, Poland.
J Chromatogr B Biomed Appl. 1994 Oct 14;660(2):409-11. doi: 10.1016/0378-4347(94)00291-6.
A rapid, simple and accurate HPLC method is presented for the determination of nadoxolol in human plasma. Nadoxolol from plasma was successfully purified using an Adsorbex column. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column with methanol-acetonitrile-phosphate buffer (pH 3.3) (70:20:10) as the mobile phase. Detection was carried out at 254 nm. The method was tested for linearity (from 5 to 25 micrograms/ml), recovery (85%) and precision (C.V. = 4.5%).
