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来自感染了猬迭宫绦虫的BALB/c雄性小鼠的纯化腹膜嗜酸性粒细胞群体的表面标志物。

Surface markers of a purified peritoneal eosinophil population from Mesocestoides corti-infected BALB/c male mice.

作者信息

Hogarth P M, Cruise K M, McKenzie I F, Mitchell G F

出版信息

J Immunol. 1980 Jan;124(1):406-11.

PMID:6153100
Abstract

Eosinophils of approximately 95% purity were prepared from the peritoneal cavities of BALB/c male mice infected with larval cestode, Mesocestoides corti. The alloantigenic surface marker phenotype of this cell population was shown to be H-2+Ly4+Ly5+Lyt-1-,2-,3-Ly-6-,7-Ia-Thy-1-TL-. Two of four anti-Lyt-2 sera were positive when tested on purified eosinophils by using the Staphylococcus aureus protein A sheep erythrocyte rosetting method, but absorption studies indicated that this reaction was not due to anti-Lyt-2 antibodies. Eosinophils are therefore Lyt-2-, although some Lyt-2 sera contain additional eosinophil reactive antibodies. A proportion (20 to 40%) of the population of eosinophils was positive for the Fc receptor, but all were negative for the C3 receptor and for surface immunoglobulin.

摘要

从感染幼虫绦虫——中殖孔绦虫的BALB/c雄性小鼠腹腔中制备纯度约为95%的嗜酸性粒细胞。该细胞群体的同种异体抗原表面标志物表型显示为H-2+Ly4+Ly5+Lyt-1-、2-、3-Ly-6-、7-Ia-Thy-1-TL-。当使用金黄色葡萄球菌蛋白A羊红细胞玫瑰花结法在纯化的嗜酸性粒细胞上进行检测时,四份抗Lyt-2血清中有两份呈阳性,但吸收研究表明该反应并非由抗Lyt-2抗体引起。因此,嗜酸性粒细胞为Lyt-2-,尽管一些抗Lyt-2血清含有额外的嗜酸性粒细胞反应性抗体。嗜酸性粒细胞群体中有一部分(20%至40%)Fc受体呈阳性,但所有细胞的C3受体和表面免疫球蛋白均为阴性。

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引用本文的文献

1
IgG and complement receptors on purified mouse eosinophils and neutrophils.纯化小鼠嗜酸性粒细胞和中性粒细胞上的IgG及补体受体
Immunology. 1981 Aug;43(4):779-86.