Dombro R S, Hutson D G
Clin Chim Acta. 1980 Jan 31;100(3):231-7. doi: 10.1016/0009-8981(80)90271-5.
A method has been developed for the separation of 5-hydroxyindoleacetic acid from substances in urine and cerebrospinal fluid which interfere with its fluorometric determination. This involves the use of anion exchange column chromatography with elution of the 5-hydroxyindoleacetic acid by 3 mol/l formic acid in 60% methanol. The indole acid is then assayed fluorometrically after reaction with o-phtalaldehyde. Urinary 5-hydroxyindoleacetic acid values are reported for patients with stable cirrhosis and hepatic encephalopathy and compared with those found in control subjects without liver disease.
已开发出一种从尿液和脑脊液中干扰5-羟吲哚乙酸荧光测定的物质中分离该物质的方法。这涉及使用阴离子交换柱色谱法，用60%甲醇中的3mol/L甲酸洗脱5-羟吲哚乙酸。然后在与邻苯二甲醛反应后，用荧光法测定吲哚酸。报告了肝硬化稳定期和肝性脑病患者的尿5-羟吲哚乙酸值，并与无肝病的对照受试者的值进行了比较。
