Miller S D, Conlon P J, Sy M S, Moorhead J W, Colón S, Grey H M, Claman H N
J Immunol. 1980 Mar;124(3):1187-93.
Unresponsiveness to DNFB contact sensitivity induced by DNP-modified lymphoid cells (DNP-LC) is mediated by two separable pathways: a rapidly induced, long lasting inhibition of reactive T cell clones (donor tolerance), and a transient period of suppressor T cell (Ts) activity. The present report has examined the nature of the hapten-modified determinants responsible for the induction of these pathways by utilizing soluble DNP-LC cell lysate preparations as tolerogens. The results indicate that both DNP-modified MHC and non-MHC encoded determinants can mediate donor tolerance 7 days after tolerization. On the other hand, the induction of Ts requires DNP-modified MHC determinants, since DNP-LC lysates passed over lentil lectin or specific anti-H-2 immunoabsorbent columns lost their ability to induce Ts. Additional experiments showed that the injection of DNP-LC lysate compatible with the recipient strain at the H-2K and H-2D region of the MHC was sufficient for the induction of Ts. We propose that Ts induction involves the direct presentation of DNP-H-2 determinants to Ts precursors, whereas the induction of donor tolerance may involve host processing and presentation of DNP-modified membrane determinants in conjunction with host MHC structures.
对由二硝基苯基修饰的淋巴细胞（DNP-LC）诱导的二硝基氟苯接触敏感性无反应性是由两条可分离的途径介导的：对反应性T细胞克隆的快速诱导、持久抑制（供体耐受），以及抑制性T细胞（Ts）活性的短暂时期。本报告通过利用可溶性DNP-LC细胞裂解物制剂作为耐受原，研究了负责诱导这些途径的半抗原修饰决定簇的性质。结果表明，在耐受诱导7天后，DNP修饰的MHC和非MHC编码决定簇均可介导供体耐受。另一方面，Ts的诱导需要DNP修饰的MHC决定簇，因为通过扁豆凝集素或特异性抗H-2免疫吸附柱的DNP-LC裂解物失去了诱导Ts的能力。额外的实验表明，在MHC的H-2K和H-2D区域注射与受体菌株相容的DNP-LC裂解物足以诱导Ts。我们提出，Ts的诱导涉及将DNP-H-2决定簇直接呈递给Ts前体，而供体耐受的诱导可能涉及宿主对DNP修饰的膜决定簇的加工和呈递，并与宿主MHC结构相结合。
