Geryk J, Mazo A, Svoboda J, Hlozánek I
Folia Biol (Praha). 1980;26(1):34-41.
The replication of transformation-defective mutants of the Prague strain of Rous sarcoma virus subgroup C was studied using roller cultures. Under such conditions, 10(5)--10(6) infectous units of virus per 0.2 ml were produced, as revealed in both the reverse transcriptase and 16Q complementation tests. A new td daPR-RSV-C mutant was isolated from duck-adapted PR-RSV-C. This mutant replicated in roller cultures with equal efficiency as the original td PR-RSV-C. It was verified that td daPR-RSV-C does not transform chicken fibroblasts, is not oncogenic for 3-week-old chickens and has subgroup C host-range specificity. Both td mutants replicate in duck cells and reach the same titres.
利用滚瓶培养研究了劳斯肉瘤病毒C亚群布拉格株转化缺陷型突变体的复制情况。在这种条件下，逆转录酶和16Q互补试验均显示，每0.2 ml产生10(5)--10(6)个感染性病毒单位。从鸭适应型PR-RSV-C中分离出一种新的td daPR-RSV-C突变体。该突变体在滚瓶培养中的复制效率与原始的td PR-RSV-C相同。经证实，td daPR-RSV-C不能转化鸡成纤维细胞，对3周龄鸡无致癌性，具有C亚群宿主范围特异性。两种td突变体都能在鸭细胞中复制并达到相同的滴度。
