Watson R R, Antal M
J Nutr. 1980 Apr;110(4):771-7. doi: 10.1093/jn/110.4.771.
Chronic protein insufficiency was achieved in rats with an 8% protein diet initiated at weaning. The total activity and activity per milliliter of the principal salivary protein, amylase, were significantly decreased at day 66. The amount of total protein per milliliter of saliva and total protein in stimulated saliva collected during 30 minutes was decreased in the malnourished rats. The levels of immunoglobulin G (IgG) and aminopeptidase in saliva were not affected by dietary status. Prolonged malnutrition on the low-protein, high-starch diet resulted in an apparent stimulation or induction of salivary amylase and protein secretion by day 110. Total protein collected and milligram of protein per milliliter of saliva were very similar in the malnourished and control rats at day 110. Salivary amylase activity in the malnourished rats was significantly greater than in the saliva of controls. The initial decrease in the principal salivary protein, amylase, and in total protein followed by a significant increase with prolonged stimulation by low-protein and high-starch diets suggests that early protein deficiency retards synthesis of amylase and that a high-starch diet may induce salivary amylase production.
通过在大鼠断奶时开始给予8%蛋白质饮食，使其出现慢性蛋白质不足。在第66天时，主要唾液蛋白淀粉酶的总活性和每毫升活性显著降低。营养不良大鼠每毫升唾液中的总蛋白量以及30分钟内收集的刺激唾液中的总蛋白量均减少。唾液中免疫球蛋白G（IgG）和氨肽酶的水平不受饮食状态的影响。在低蛋白、高淀粉饮食下长期营养不良，到第110天时导致唾液淀粉酶和蛋白质分泌明显受到刺激或诱导。在第110天时，营养不良大鼠收集的总蛋白和每毫升唾液中的蛋白毫克数与对照大鼠非常相似。营养不良大鼠的唾液淀粉酶活性显著高于对照大鼠的唾液。主要唾液蛋白淀粉酶和总蛋白最初下降，随后在低蛋白和高淀粉饮食的长期刺激下显著增加，这表明早期蛋白质缺乏会阻碍淀粉酶的合成，而高淀粉饮食可能诱导唾液淀粉酶的产生。
