膜活性物质对培养的主动脉内皮细胞表面形态的影响。

Influence of membrane active substances on cell surface morphology of cultured aortic endothelial cells.

Csonka E, Bernolák B, Koch A S, Jellinek H

Acta Morphol Acad Sci Hung. 1977;25(2-3):147-59.

Abstract

The changes occurring on the surface of cultured aortic endothelial cells under the influence of exogenous cholesterol, lysolecithin, or cholesterol + lysolecithin were pursued by scanning and transmission electron microscopy. 2. Both compounds were found to cause surface changes under different quantitative relations, as shown by scanning electron microscopy of unfixed preparations. The changes were less significant in preparations fixed prior to the scanning--or transmission electron microscopic examination. 3. Other cell lines maintained in this laboratory and similarly treated with cholesterol, were found to be less responsive than the endothelial cells. 4. Cholesterol content was determined by thin layer chromatography in six different cell lines, before and after the addition of cholesterol. Only the endothelial cells showed a notable rise of cholesterol content after treatment. This fact may confirm our finding that cholesterol induced morphological changes were demonstrable only in the endothelial cells.

摘要

通过扫描电子显微镜和透射电子显微镜观察了外源性胆固醇、溶血卵磷脂或胆固醇+溶血卵磷脂作用下培养的主动脉内皮细胞表面发生的变化。2. 如对未固定标本进行扫描电子显微镜观察所示，发现这两种化合物在不同的定量关系下均会引起表面变化。在进行扫描或透射电子显微镜检查之前固定的标本中，变化不太明显。3. 本实验室保存的其他细胞系，经类似胆固醇处理后，发现其反应性低于内皮细胞。4. 在添加胆固醇前后，通过薄层色谱法测定了六种不同细胞系中的胆固醇含量。仅内皮细胞在处理后显示胆固醇含量显著升高。这一事实可能证实了我们的发现，即胆固醇诱导的形态变化仅在内皮细胞中可见。