Biochemical properties of alkaline phosphatase isoenzymes and their practical significance.

Certain physiochemical and biochemical properties of serum alkaline phosphatase isoenzymes are described concisely. Differences in their properties can be utilized in practice for the qualitative and quantitative determination of isoenzyme activities in sera in pathological states. Some of the methodological requirements of this differentiation by electrophoretic separation and inactivation-inhibition methods are evaluated.