Atkison P R, Weidman E R, Bhaumick B, Bala R M
Endocrinology. 1980 Jun;106(6):2006-12. doi: 10.1210/endo-106-6-2006.
Confluent cultures of normal diploid WI-38 human embryonic lung fibroblasts released somatomedin (SM)-like activity into their incubation medium during culture in serum-free medium. This postculture medium (conditioned medium) stimulated cell division in these same cultured WI-38 fibroblasts and 35SO4 uptake by hypophysectomized rat cartilage in vitro. The conditioned medium also contained immunoreactive SM (IRSM) activity which yielded parallel dose-response curves to human serum in a RIA for SM. The IRSM activity measured in conditioned medium was not the artifactual result of effects of possible SM-binding proteins or proteolytic enzymes in conditioned medium. These studies suggest that cultured WI-38 fibroblasts produce and release SM-like activity which has SM-like biological activity and is immunoreactive with a basic SM purified from human plasma Cohn fraction and having similarity with SM-C and insulin-like growth factor-I. Human GH appears to stimulate production and release of IRSM activity by these cells.
正常二倍体WI-38人胚肺成纤维细胞的汇合培养物在无血清培养基中培养期间，向其培养液中释放了类生长调节素（SM）活性。这种培养后的培养基（条件培养基）刺激了这些相同培养的WI-38成纤维细胞的细胞分裂以及垂体切除大鼠软骨在体外对35SO4的摄取。条件培养基还含有免疫反应性SM（IRSM）活性，在SM的放射免疫分析中，它对人血清产生平行的剂量反应曲线。在条件培养基中测得的IRSM活性并非条件培养基中可能存在的SM结合蛋白或蛋白水解酶作用的人为结果。这些研究表明，培养的WI-38成纤维细胞产生并释放具有SM样生物活性的类SM活性，且与从人血浆Cohn组分中纯化的碱性SM具有免疫反应性，与SM-C和胰岛素样生长因子-I相似。人GH似乎刺激这些细胞产生并释放IRSM活性。
