Ledvina M, Kraml J, Kolínská J
Acta Univ Carol Med Monogr. 1977(79 Pt 3):165-71.
The authors studied the separation of glucoamylase and beta-galactosidases from the interstinal mucosa of young rats by affinity chromatography. They tested the following chromatographic materials: p-aminophenyl-beta-D-thioglucoside bound to Sepharose 4B via hexamethylenediamine, gluconate and galactonate bound in different ways to Sepharose 4B and phlorizin bound by an azo-coupling reaction to a spacer attached to Sepharose 4B. The conditions of the adsorption of glycosidases to these materials and their subsequent elution were studied. Using chromatography on Sepharose 4B with a beta-thioglucoside affinant, we succeeded in purifying lactase preparation so that, in electrophoresis on polyacrylamide gel, it formed a single zone identical with 1-naphthyl-beta-glucosidase activity.
作者们通过亲和色谱法研究了从幼鼠肠道黏膜中分离葡糖淀粉酶和β-半乳糖苷酶的方法。他们测试了以下色谱材料：通过六亚甲基二胺与琼脂糖4B结合的对氨基苯基-β-D-硫代葡糖苷、以不同方式与琼脂糖4B结合的葡萄糖酸盐和半乳糖酸盐，以及通过偶氮偶联反应与连接到琼脂糖4B的间隔基结合的根皮苷。研究了糖苷酶在这些材料上的吸附条件及其随后的洗脱条件。使用带有β-硫代葡糖苷亲和剂的琼脂糖4B进行色谱分析，我们成功地纯化了乳糖酶制剂，使得在聚丙烯酰胺凝胶电泳中，它形成了一个与1-萘基-β-葡糖苷酶活性相同的单一区带。
