Yagi M J, King N W, Bekesi J G
J Virol. 1980 Apr;34(1):225-33. doi: 10.1128/JVI.34.1.225-233.1980.
The effects of exogenous mouse interferon on the MJY-alpha mammary tumor cell line chronically infected with mouse mammary tumor virus (MMTV) were examined. Interferon at concentrations of 25 to 2,000 IU/ml in culture medium did not alter the growth rate or morphology of the cell layers. Electron microscopic examination of interferon-treated cells indicated a decrease in the numbers of A-type and budding B-type particles of MMTV. However, the levels of extracellular MMTV virions in the culture supernatants were not significantly reduced. Profiles of MMTV glycoproteins and nonglycosylated polypeptides obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virions purified from interferon-treated cultures revealed increases in the relative levels of the 60,000-dalton glycoprotein, gp60.
研究了外源性小鼠干扰素对长期感染小鼠乳腺肿瘤病毒（MMTV）的MJY-α乳腺肿瘤细胞系的影响。培养基中浓度为25至2000 IU/ml的干扰素未改变细胞层的生长速率或形态。对经干扰素处理的细胞进行电子显微镜检查表明，MMTV的A型和出芽B型颗粒数量减少。然而，培养上清液中细胞外MMTV病毒粒子的水平并未显著降低。通过对从经干扰素处理的培养物中纯化的病毒粒子进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳获得的MMTV糖蛋白和非糖基化多肽图谱显示，60000道尔顿糖蛋白gp60的相对水平有所增加。
