Silverman A J, Gadde C A, Zimmerman E A
Neuroendocrinology. 1980 May;30(5):285-90. doi: 10.1159/000123016.
Using radioautography and immunocytochemistry, we investigated differences in 3H-cytidine incorporation into RNA in vasopressin or neurophysin-positive neurons in the rat hypothalamus following adrenalectomy. Control animals and those that had been bilaterally adrenalectomized for 2 weeks received 0.3 mCi 3H-cytidine subcutaneously and were decapitated 1 h following injection. Brains were prepared for combined radioautography and immunocytochemistry. Incorporation was estimated by counting silver grains over immunoreactive neurons. The neurons of the paraventricular nucleus (PVN) showed a consistent and significant increase in the amount of incorporation of label 2 weeks after bilateral adrenalectomy. There was no change in incorporation in neurons of either the supraoptic or suprachiasmatic nuclei. These findings suggest that the vasopressin PVN neurons, some of which project to the zona externa of the median eminence, are activated under conditions of adrenalectomy.
我们使用放射自显影术和免疫细胞化学方法，研究了肾上腺切除术后大鼠下丘脑加压素或神经垂体素阳性神经元中3H-胞苷掺入RNA的差异。对照动物和双侧肾上腺切除2周的动物皮下注射0.3 mCi 3H-胞苷，并在注射后1小时断头。制备大脑用于放射自显影术和免疫细胞化学联合检测。通过计数免疫反应性神经元上的银颗粒来估计掺入量。双侧肾上腺切除2周后，室旁核（PVN）的神经元显示标记掺入量持续且显著增加。视上核或视交叉上核的神经元掺入量没有变化。这些发现表明，一些投射到正中隆起外侧带的加压素PVN神经元在肾上腺切除条件下被激活。
