Kobes R D, Wyatt R J, Neckers L M
J Pharmacol Methods. 1980 Jun;3(4):305-10. doi: 10.1016/0160-5402(80)90072-8.
A rapid and sensitive assay for the measurement of monoamine oxidase (MAO) activity was developed. This method utilizes high pressure liquid chromatography with fluorescence excitation at 280 nm and detection of 330 nm at pH 5.0 of indoleacetic acid and 5-hydroxyindoleacetic acid, the deaminated products of two substrates for MAO, tryptamine, and serotonin, respectively. The assay allows for the complete separation of metabolites from either of the two substrates. The method has been used to determine MAO activity in the frontal cortex and caudate nucleus of rat brain using tryptamine and serotonin as substrates. The ease and rapidity of this assay make it sutable for use where routine enzyme determinations are required.
开发了一种用于测量单胺氧化酶（MAO）活性的快速灵敏的检测方法。该方法利用高压液相色谱法，在280nm处进行荧光激发，并在pH 5.0条件下检测吲哚乙酸和5-羟基吲哚乙酸，它们分别是MAO的两种底物色胺和血清素的脱氨基产物。该检测方法能够将两种底物中的代谢物完全分离。该方法已用于以色胺和血清素为底物测定大鼠脑额叶皮质和尾状核中的MAO活性。这种检测方法的简便性和快速性使其适用于需要进行常规酶测定的场合。
