乙硫氨酸和β-二乙氨基乙基二苯基丙基乙酸酯对大鼠肝脏RNA和蛋白质合成的抑制作用及其被孕烯醇酮-16α-腈逆转的情况。

Inhibition by ethionine and beta-diethylaminoethyl diphenylpropylacetate of RNA and protein synthesis in the rat liver, and its reversal by pregnenolone-16 alpha-carbonitrile.

作者信息

Lykissas E D, Kourounakis P

出版信息

Arzneimittelforschung. 1980;30(4):630-2.

Abstract

The effect of pregnenolone-16 alpha-carbonitrile (PCN) on female Sprague-Dawley rats was studied in conjunction with ethionine and beta-diethylaminoethyl diphenylpropylacetate (DADP). The cyanosteroid virtually abolished in inhibitory action of ethionine on orotic acid and leucine uptake by liver microsomes. When PCN was given conjointly with DADP, it completely overshadowed the latter's effect on leucine uptake. DADP was found to act as an inhibitor of the translational mechanism 5 h after its administration, while it exerted a stimulatory influence on the transcriptional mechanism after the same time period.

摘要

孕烯醇酮-16α-腈（PCN）对雌性斯普拉格-道利大鼠的作用，与乙硫氨酸和β-二乙氨基乙基二苯基丙基乙酸酯（DADP）一起进行了研究。这种氰基类固醇实际上消除了乙硫氨酸对肝微粒体乳清酸和亮氨酸摄取的抑制作用。当PCN与DADP联合给药时，它完全掩盖了后者对亮氨酸摄取的影响。发现DADP在给药5小时后作为翻译机制的抑制剂起作用，而在同一时间段后它对转录机制发挥刺激作用。