Lykissas E D, Kourounakis P, Selye H
Res Commun Chem Pathol Pharmacol. 1978 Jan;19(1):173-6.
Pregnenolone-16alpha-carbonitrile (PCN) enhanced adenyl cyclase activity in the liver cells of female Sprague-Dawley rats. Injection of 14C-PCN and fractionation of hepatocytes into nuclei, mitochondria, total ribosomes, endoplasmic reticulum (ER) membranes, cytosol and plasma membranes revealed that after 4 h there was a preferential localization of the cyanosteroid in ER membranes. Total ribosomes and cytosolar proteins seemed to contain less PCN after 4 h, the optimal time for maximal penetration and intracellular establishment. On the other hand, PCN was localized preferentially in plasma membranes after 2 h and this diminished with time. Only trace amounts of the cyanosteroid were found in the nuclei and mitochondria, from the time of its intracellular introduction to the time of its apparent removal from the hepatocyte.
孕烯醇酮 -16α- 腈（PCN）增强了雌性斯普拉格 - 道利大鼠肝细胞中的腺苷酸环化酶活性。注射14C - PCN并将肝细胞分离成细胞核、线粒体、总核糖体、内质网（ER）膜、细胞质溶胶和质膜后发现，4小时后氰基类固醇优先定位于ER膜中。4小时后，总核糖体和细胞质溶胶蛋白似乎含有的PCN较少，4小时是最大渗透和细胞内建立的最佳时间。另一方面，PCN在2小时后优先定位于质膜中，并且随着时间的推移这种定位减少。从氰基类固醇细胞内引入到从肝细胞中明显清除的这段时间内，仅在细胞核和线粒体中发现痕量的氰基类固醇。
