Tayler R, Orwell R L, Piper D W
Digestion. 1977;16(1-2):40-7. doi: 10.1159/000198053.
In an attempt to determine the significance of increased beta-glucuronidase content of gastric juice of patients with gastric carcinoma, gastric mucosal cells were exposed, in organ culture technique, to a known gastric carcinogen and indices of carcinogenic activity were studied in the ambient fluid and in the mucosal cells. Isotopic methods were used to determine cell viability. Indices of carcinogenic activity in the ambient fluid were beta-glucuronidase and lactate production and changes in the LDH isoenzyme pattern of the homogenates of the exposed cells were also studied. Incubation with the carcinogen resulted in increased production of beta-glucuronidase and lactate, suggesting the increased beta-glucuronidase activity in the gastric juice of patients with gastric cancer indicates malignancy.
为了确定胃癌患者胃液中β-葡萄糖醛酸酶含量增加的意义，采用器官培养技术将胃黏膜细胞暴露于一种已知的胃癌致癌物中，并研究周围液体和黏膜细胞中的致癌活性指标。使用同位素方法测定细胞活力。周围液体中的致癌活性指标为β-葡萄糖醛酸酶和乳酸生成，同时也研究了暴露细胞匀浆中乳酸脱氢酶同工酶模式的变化。与致癌物孵育导致β-葡萄糖醛酸酶和乳酸生成增加，这表明胃癌患者胃液中β-葡萄糖醛酸酶活性增加表明存在恶性肿瘤。
