Wijdenes J, van Minnen J, Boer H H
Cell Tissue Res. 1980;210(1):47-56. doi: 10.1007/BF00232140.
Neurosecretion was studied in the central ganglia of three terrestrial pulmonates (Deroceras reticulatum, Arion hortensis, and Helix aspersa) with the alcian blue-alcian yellow (AB/AY) staining method. A number of neurosecretory cell types were distinguished in all three species (D. reticulatum: 9, A. hortensis: 11, H. aspersa: 13) With AB/AY the cells stain different shades of green and yellow. The histochemical differences reflect ultrastructural differences: the cell types contain elementary granules of different size and appearance. Some cell types form distinct groups of up to 50 cells, while others occur in smaller groups or as single cells. Neurosecretory axons could only be traced in a few cases to the probable neurohaemal areas. Based on the following criteria: (1) staining properties of cells (2) size of cells, (3) position of cells in the ganglia, it appears that the neurosecretory systems of the three species show great similarity, although some differences are apparent.
采用阿尔辛蓝 - 阿尔辛黄（AB/AY）染色法，对三种陆生肺螺类动物（网纹蛞蝓、庭园蛞蝓和散大蜗牛）的中枢神经节中的神经分泌进行了研究。在这三种物种中均鉴别出了多种神经分泌细胞类型（网纹蛞蝓：9种；庭园蛞蝓：11种；散大蜗牛：13种）。用AB/AY染色后，细胞呈现出不同深浅的绿色和黄色。组织化学差异反映了超微结构差异：这些细胞类型含有大小和外观各异的基本颗粒。一些细胞类型形成了多达50个细胞的明显群体，而其他细胞则以较小群体或单个细胞的形式出现。仅在少数情况下能追踪到神经分泌轴突至可能的神经血器官区域。基于以下标准：（1）细胞的染色特性；（2）细胞大小；（3）细胞在神经节中的位置，这三种物种的神经分泌系统似乎具有很大的相似性，尽管也存在一些明显差异。
